Lagroye, Anane R, Wettring BA, Moros EG, et al. (2004 b)
Lai and Singh (1995, 1996) reported that 2450 MHz microwaves induced DNA damage in brain cells of exposed rats. The present authors attempted to confirm these findings, using identical methodology. They used the same alkaline assay procedure as Lai and Singh to measure DNA damage, but also did two further assay procedures. They used the Singh method with and without proteinase K. The original Singh procedure used proteinase K, which removes nuclear proteins. This could affect the assay results. The present authors also did parallel assays using an alternative method by Olive (1992) that uses a pH slightly lower than that in the Singh method.
Rats were exposed (and control rats sham-exposed) to 2450 MHz pulsed microwaves for 2 hours at a whole-body SAR of 1.2 W/kg. Four hours after exposure the rats were sacrificed and the brain removed for the assay procedure. Some of the rats were exposed to gamma radiation just before being killed. This was designed to produce positive controls for the experiments. The researchers doing the assays were blinded as to the exposure status of the animals.
Significant DNA damage was seen in the brain cells of rats exposed to gamma radiation using all versions of the assay procedure. However, no DNA damage was observed in the cells of rats exposed to the 2450 MHz microwaves in either the Singh or the Olive assay, whether or not proteinase K was included in the assay.
Hence, the authors could not confirm the results of Lai and Singh.