Autores

Lee K-Y, Kim BC, Han N-K, Lee Y-S, Kim T, Yun J-H, Kim N, Pack J-K, Lee J-S. Effects of combined radiofrequency radiation exposure on the cell cycle and its regulatory proteins.  Bioelectromagnetics. 28 Oct, 2010. Ahead of print.

Background
The excessive use of electronic devices has raised public health concerns about exposure to radiofrequency radiation (RF).  All types of cells undergo a cycle that leads to cell division.  There are several phases to this process and many different proteins are involved in the different phases. Disruption of this cycle due to stressors and DNA damage is thought to be one of the determinants that lead to disease, including cancer.  This study investigates whether single or multiple RF exposures have any effect on the proteins involved in the cell cycle.

Objective
The objective of this study was to determine if exposure to single or combined RF radiation alters any major proteins involved in normal cell cycle division.

Methods
For this study, four set of experiments were done with cells (breast cancer cell line) in Petri dishes.  The first set of cells were placed in an exposure chamber and exposed to 837 MHz radiation for 1 hour. The second set of cells were also placed in the exposure chamber and exposed to both 837 MHz and 1,950 MHz radiation for one hour.  The third set of cells were considered the negative control and placed in the exposure chamber, but were not exposed to any radiation. The fourth group were considered the positive control group and were exposed to gamma radiation, which has been previously shown to disrupt cell cycle division.  All cells were placed in a bath in the exposure chamber so that the temperature would be the same for all the experiments.  After exposure, the cells were returned to their incubators and cells were isolated at 0, 2, 10, 24, or 48 hour time points.  Proteins were extracted from the cells and a number of protein amounts were analyzed using standard detection methods.

Results
The negative control cells and the cells exposed to the single or combined RF did not show any alterations in the cell cycle proteins analyzed.  The positive control where cells were exposed to ionizing radiation showed changes in cell cycle proteins as expected. 

Interpretation and Limitations
The authors discussed that even though higher doses of radiation were used in this study than people are normally exposed to, there was no change in the cellular cycle components. This study indicates that even simultaneous exposure from two different frequencies still had no effect on cell cycle.  However, the authors did state that further studies need to be done in order to examine continuous and long-term effects of non-ionizing radiation on cell cycle division.

Conclusion
This study indicates that single or combined acute RF exposure does not disrupt proteins involved in cell cycle division.



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