Hirose H, Sakuma N, Kaji N, Nakayama K, et al. (2007)
The aim of the study was to assess the effect of RFR on heat shock proteins. The authors studied the effects of RFR exposure on two cell lines - human glioblastoma A172, and human IMR-90 fibroblast. The cells were exposed to the W-CDMA cellular system, which is one of the component systems of the IMT-2000 system, and to continuous wave (CW) radiation.
The A172 cells and IMP-90 fibroblasts were exposed to W-CDMA radiation at SARs of 0.08 and 0.8 W/kg for 2 hours. Next, the authors defined the cell doubling time of the A172 and IMR-90 cells, cultivated in 35mm culture dishes, to be 20-24 and 24-28 hours respectively. A172 cells were exposed to W-CDMA RFR at SARs of 0.08, 0.25, and 0.0 W/kg, and to CW RFR at 0.08 W/kg, for 24 or 48 hours (equalling one or two doubling times of the cells). IMR-90 cells were exposed to W-CDMA at 0.08 and 0.8 W/kg, and CW at 0.08 W/kg, for 28 hours, which is one doubling time of the cell. Sham exposure was also used The RF field exposure was assessed in a blind manner. HSP27 phosphorylation was assessed by bead-based multiplex assays, and gene expression of HSPs was assessed by DNA Chip analysis.
No differences were noted between exposed and sham-exposed cells
in phosphorylation levels or in gene expression of HSPs.